Plasmid
GeneArt pG

Part:BBa_J70003:Design

Designed by: Randy Rettberg   Group: Knight Lab   (2008-01-16)

Geneart Cloning Plasmid pGA4


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 701
    Illegal PstI site found at 707
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 701
    Illegal PstI site found at 707
    Illegal NotI site found at 737
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 701
    Illegal BamHI site found at 719
    Illegal XhoI site found at 668
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 701
    Illegal PstI site found at 707
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 701
    Illegal PstI site found at 707
    Illegal NgoMIV site found at 328
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1030


Design Notes

This plasmid needs to be checked to see where arets have actually been inserted. - Randy


The sequence for this plasmid needs to be rotated so that the suffix and prefix are at the ends.


Source

Geneart

References